Fast D-PAS

Overview

High throughput pKa measurement on GLpKa and PCA200 with D-PAS

• Fast hybrid pH/UV method saves time
• Only 4 minutes per sample!
• Easy assay set-up
• Highly automated
• pKas between 2 and 12
• Up to 50 samples in four hours
• Accurate, repeatable results
• Aqueous or cosolvent methods
• Measures labile samples before they decompose

Fast D-PAS is a software module that runs in RefinementPro 2. If you are running a GLpKa and D-PAS, Fast D-PAS software will enable you to measure pKa values very quickly.

Titrations are done in the presence of a unique linear buffer solution. This allows the pH electrode to reach a stable value very quickly after each addition of titrant.

Fast D-PAS measures pKa for water-soluble samples in four minutes. Water-insoluble samples are measured in water-solvent mixtures using a Multisweep titration. A sample is prepared in water plus buffer plus cosolvent, and titrated with base from low to high pH. At the end of the first titration, the solution is titrated with acid to low pH, then back to high pH, and so on. Each titration dilutes the cosolvent, and additional water can be added between titrations if required. It takes less than 10 minutes to do a 3-titration Multisweep between pH 2 and 12, and the cosolvent can be diluted significantly, say from 40% to 20%. A Yasuda-Shedlovsky extrapolation to the aqueous pKa based on 3 points is plotted automatically. Reference spectra for Fast D-PAS experiments are obtained from a Buffer Calibration Titration. In this procedure, a blank solution is titrated over the full pH range, and the UV spectra are saved after each addition of titrant. This procedure provides reference spectra of the highest quality.

Literature

Five publications since 2005.
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Certificates of Conformity

Material Safety Data Sheets