Sirius ProfilerSGA - High Throughput pKa measurement

Profiler SGA

Overview

For high throughput pKa measurement from microtitre plates.

Fully automated physicochemical profiling
Multi-wavelength spectrophotometric technique
Open access option
Automated data handling and processing
Full computer control
Micro-plate format
Measures below 0.01mM concentration
300 assays in 24 hours

The Sirius ProfilerSGA uses multi-wavelength UV spectroscopy and a linear 2-12pH unit gradient for truly rapid pKa determination. Taking samples directly from stock solutions and working on a microtitre tray format, ProfilerSGA automatically prepares the samples by diluting to the required concentration. Each solution is then injected directly into a pH gradient stream, mixed and passed through the quartz flow cell, allowing pKas to be measured in just two minutes. Results are calculated by SGARefine 3 software.

Data can be reported in a number of user-definable formats, including spreadsheets and printouts. The system has configurable security options that allow for open access. Quality assured standards are used to calibrate the gradient and verify overall system performance.

Technical specificiation

Liquid handling

Robotic platform with X, Y & Z single probe

Dispensers

3 unit, minimum increment of 0.4 microlitres with 5mL syringe

Degasser

4 channel vacuum unit

Tubing

FEP before dispensers, PEEK after

Gradient mixer

Stainless Steel T

Worktable

4 microtitre plate positions and 6 large vial locations

Injection port

Single point, direct injection

Detector type

Diode array, 1024 diodes

Detector wavelength rge

190 - 1000nm

Signal-to-noise

±1x10-5AU at 250nm

Flow cell

8 microlitre volume, quartz windows

Path length

10mm

Fibre material

Quartz

Lamp

Deuterium

Lamp lifetime

2000h (500h warranty)

Sample format

20mL of 10mM DMSO

Typical sample concentration range

10-4 - 10-6M

pKa detection range

2.0 - 12.0

Services required

One mains power inlet 240V

PC specifications

One free PCI expansion slot for data collection card One serial comms ports

Graphics card that supports OpenGL

Connection to the PC

Cable from diode array to data collection board 800mm maximum length: PC must be next to the Profiler

Cable from PC to robotic unit

Software

Windows NT v 4.0 operating system or Windows 2000
Microsoft Office 2000
Explorer 5 or greater
(Networking TCP/IP) ProfilerREFINE
ProfilerCONTROL

Reagent requirements

Acidic buffer component

Basic buffer component
Deionised water
Calibration Standard 1

Calibration Standard 2

Dimensions

600 W x 550 D x 970 H mm

Weight

70kg

Literature

Electronic copies of our ProfilerSGA brochure and technical specifications are available for download:

> ProfilerSGA Brochure
> ProfilerSGA Technical Specifications